Experimental study on treatment effect of MET on renal injuries in gas explosion

doi:10.16265/j.cnki.issn1003-3033.2022.10.2123

Abstract

Abstract:

In order to explore characters of kidney injuries caused by gas explosion and treatment effect of MET as well as its possible action mechanism， rat samples were divided into normal control group （NC group）， kidney injury caused by gas explosion group （SW group） and kidney injury caused by gas explosion treated with MET group randomly. Then， kidney injury model of rats was built by utilizing actual roadway， renal functions were tested， histological changes of kidney and express changes of apoptosis associated molecular were observed， and those of AMPK and PGC-1α were detected. The results show that kidney injuries are normally expressed as significant congestion and edema in renal corpuscle and kidney tubules along with kidney disfunction. MET can improve renal function and decrease damages probably through activating AMPK and PGC-1α， thus inhibiting apoptosis of renal tubular epithelial cells.

Key words: metformin（MET）, gas explosion, acute kidney injury, AMP-activated protein kinase （AMPK）, peroxidase proliferator-activated receptor gamma coactivator 1α （PGC-1α）

TIAN Linqiang, LAI Feng, ZHANG Yue, WENG Xiaogang, YAO Sanqiao, REN Wenjie. Experimental study on treatment effect of MET on renal injuries in gas explosion[J]. China Safety Science Journal, 2022, 32(10): 207-213.

Figures/Tables 4

References 25

[1]	任文杰, 孟伟正, 李嵩岳, 等. 346例瓦斯爆炸烧伤伤情特点[J]. 中华创伤杂志, 2007, 23(11): 870-873.
	REN Wenjie, MENG Weizheng, LI Songyue, et al. Characteristics of burn injury induced by gas explosion in 346 cases[J]. Chinese Journal of Trauma, 2007, 23(11): 870-873.
[2]	王杨, 王锦文, 关大庆, 等. 瓦斯爆炸烧伤患者救治[J]. 创伤与急危重病医学, 2014, 2(2): 105-107.
[3]	白俊清, 刘卓, 闫金银, 等. 我国瓦斯爆炸伤害医疗救治的现状与思考(二)[J]. 中国煤炭工业医学杂志, 2008, 11(9): 1436-1438.
	BAI Junqing, LIU Zhuo, YAN Jinyin, et al. Status quo and thinking of injury insurance of gas explosion in China[J]. Chinese Journal of Coal Industry Medicine, 2008, 11(9): 1436-1438.
[4]	王海洋, 赵树磊, 陈祥, 等. 我国隧道瓦斯事故统计及影响因素分析[J]. 中国安全科学学报, 2021, 31(4): 34-40. doi: 10.16265/j.cnki.issn1003-3033.2021.04.005
	WANG Haiyang, ZHAO Shulei, CHEN Xiang, et al. Statistics and influencing factors analysis of tunnel gas accidents in China[J]. China Safety Science Journal, 2021, 31(4): 34-40. doi: 10.16265/j.cnki.issn1003-3033.2021.04.005
[5]	王志荣, 孙培培, 唐振华, 等. 密闭容器甲烷-空气混合物爆炸的尺寸效应[J]. 中国安全科学学报, 2021, 31(1): 60-66. doi: 10.16265/j.cnki.issn 1003-3033.2021.01.009
	WANG Zhirong, SUN Peipei, TANG Zhenhua, et al. Size effect of methane-air mixture explosion in closed vessel[J]. China Safety Science Journal, 2021, 31(1): 60-66. doi: 10.16265/j.cnki.issn 1003-3033.2021.01.009
[6]	杨晋杰, 林志铭, 王晓君, 等. 矿井内瓦斯爆炸烧伤1 087例临床分析[J]. 江西煤炭科技, 2002(3): 52-54.
[7]	王建军, 谌煜. 140例瓦斯爆炸烧伤救治分析[J]. 湖北民族学院学报:医学版, 2005, 22(4): 27-29.
	WANG Jianjun, CHEN Yu. Cure analysis of 140 burn cases of gas explosion[J]. Journal of Hubei University for Nationalities :Medical Edition, 2005, 22(4):27-29.
[8]	赵俊龙, 胡传义, 郑景存, 等. 银杏达莫对体外冲击波碎石术致患者早期肾损伤的保护作用[J]. 宁夏医科大学学报, 2018, 40(5): 537-541.
	ZHAO Junlong, HU Chuanyi, ZHENG Jingcun, et al. The protective effects of ginkgo biloba against early renal injury induced by extracorporeal shock wave lithotripsy[J]. Journal of Ningxia Medical University, 2018, 40(5): 537-541.
[9]	李继清, 陈富昌, 刘刚, 等. 高压氧对体外冲击波碎石术性肾脏损伤家兔凝血功能及血细胞的影响[J]. 中国临床新医学, 2015(12): 1117-1120.
	LI Jiqing, CHEN Fuchang, LIU Gang, et al. The influence of hyperbaric on coagulation function and blood cells oxygen in rabbits with renal injury induced by ESWL[J]. Chinese Journal of Clinical New Medicine, 2015(12): 1117-1120.
[10]	ZHAN Ming, BROOKS C, LIU Fuyou, et al. Mitochondrial dynamics: regulatory mechanisms and emerging role in renal pathophysiology[J]. Kidney International, 2013, 83(4): 568-581. doi: 10.1038/ki.2012.441 pmid: 23325082
[11]	TANG Chengyuan, CAI Juan, YIN Xiaoming, et al. Mitochondrial quality control in kidney injury and repair[J]. Nature Reviews Nephrology, 2020, 17(5):Doi: 10.1038/s41581-020-00369-0. doi: 10.1038/s41581-020-00369-0
[12]	DRAKE J, WILSON R, LAKER R, et al. Mitochondria-localized AMPK responds to local energetics and contributes to exercise and energetic stress-induced mitophagy[J]. Proceedings of the National Academy of Sciences of the United States of America, 2021, 118(37):DOI: 10.1073/pnas.2025932118. doi: 10.1073/pnas.2025932118
[13]	董新文, 姚三巧, 吴卫东, 等. 真实巷道环境下瓦斯爆炸对大鼠脑神经行为的短期影响[J]. 卫生研究, 2020, 49(6): 889-894. pmid: 33413760
	DONG Xinwen, YAO Sanqiao, WU Weidong, et al. Short-term effects of gas explosion in real roadway environment on rats' brain neural behavior[J]. Journal of Hygiene Research, 2020, 49(6): 889-894. doi: 10.19813/j.cnki.weishengyanjiu.2020.06.003 pmid: 33413760
[14]	董新文, 姚三巧, 吴卫东, 等. 真实巷道环境瓦斯爆炸对大鼠急性冲击性肺损伤呼吸功能时相变化的影响[J]. 中华劳动卫生职业病杂志, 2021, 39(2): 137-142.
	DONG Xinwen, YAO Sanqiao, WU Weidong, et al. Influences of gas explosion on acute blast lung injury and time phase changes of pulmonary function in rats under real roadway environment[J]. Chinese Journal of Industrial Hygiene and Occupational Diseases, 2021, 39(2): 137-142.
[15]	BROOKS C, WEI Qingqing, CHO S G, et al. Regulation of mitochondrial dynamics in acute kidney injury in cell culture and rodent models[J]. The Journal of Clinical Investigation, 2009, 119(5): 1275-1285. doi: 10.1172/JCI37829
[16]	全娇, 江雨鹏, 卢蓉, 等. 二甲双胍对顺铂诱导急性肾损伤大鼠的治疗作用[J]. 中南大学学报:医学版, 2020, 45(11): 1355-1360.
	QUAN Jiao, JIANG Yupeng, LU Rong, et al. Therapeutic effect of metformin on rats with cisplatin induced acute kidney injury[J]. Journal of Central South University:Medical Science, 2020, 45(11): 1355-1360.
[17]	HUGHES S, JONES N, THOMAS-WRIGHT S J, et al. Shock wave lithotripsy, for the treatment of kidney stones, results in changes to routine blood tests and novel biomarkers: a prospective clinical pilot-study[J]. European Journal of Medical Research, 2020, 25(1): DOI:10.1186/s40001-020-00417-2. doi: 10.1186/s40001-020-00417-2
[18]	CHUNG J M, PARK B K, KIM J H, et al. Impact of repeated extracorporeal shock wave lithotripsy on prepubertal rat kidney[J]. Urolithiasis, 2018, 46(6): 549-558. doi: 10.1007/s00240-017-1011-0 pmid: 29119224
[19]	SHI Si, LEI Shaoqing, TANG Chaoliang, et al. Melatonin attenuates acute kidney ischemia/reperfusion injury in diabetic rats by activation of the SIRT₁/Nrf₂/HO_-1 signaling pathway[J]. Bioscience Reports, 2019, 39(1):DOI:10.1042/BSR20181614. doi: 10.1042/BSR20181614
[20]	薛晶, 杨陈, 安宁, 等. 二甲双胍非降糖肾脏保护作用的研究新进展[J]. 中国药理学通报, 2018, 34(5): 593-596.
	XUE Jing, YANG Chen, AN Ning, et al. Non-hypoglycemic effect of metformin in renal protection:a literature review[J]. Chinese Pharmacological Bulletin, 2018, 34(5): 593-596.
[21]	WANG Min, WENG Xiaodong, GUO Jia, et al. Metformin alleviated EMT and fibrosis after renal ischemia-reperfusion injury in rats[J]. Renal Failure, 2016, 38(4): 614-621. doi: 10.3109/0886022X.2016.1149770
[22]	RABINOVITCH R, SAMBORSKA B, FAUBERT B, et al. AMPK maintains cellular metabolic homeostasis through regulation of mitochondrial reactive oxygen species[J]. Cell Reports, 2017, 21(1): 1-9. doi: S2211-1247(17)31301-3 pmid: 28978464
[23]	CLARK A, PARIKH S. Targeting energy pathways in kidney disease: the roles of sirtuins, AMPK, and PGC1α[J]. Kidney International, 2020, 99(4): 828-840. doi: 10.1016/j.kint.2020.09.037 pmid: 33307105
[24]	张梦希, 董伟. 过氧化物酶体增殖物激活受体γ辅激活物1-α在急性肾损伤中的作用[J]. 肾脏病与透析肾移植杂志, 2018, 27(4): 364-368.
	ZHANG Mengxi, DONG Wei. Peroxisome proliferator-activated receptor γcoactivator 1-αin acute kidney injury[J]. Chinese Journal of Nephrology, Dialysis & Transplantation, 2018, 27(4): 364-368.
[25]	徐洋, 李文华, 郑迪, 等. 虾青素调控SIRT1/PGC-1α/NRF1信号通路减轻对比剂诱导的急性肾小管损伤[J]. 医学研究杂志, 2018, 47(11): 75-79.
	XU Yang, LI Wenhua, ZHENG Di, et al. Astaxanthin attenuates contrast-induced acute kidney injury by regulating SIRT1/PGC-1α/NRF1 signaling pathway[J]. Journal of Medical Research, 2018, 47(11): 75-79.