二甲双胍改善瓦斯爆炸肾损伤的试验研究

doi:10.16265/j.cnki.issn1003-3033.2022.10.2123

中国安全科学学报 ›› 2022, Vol. 32 ›› Issue (10): 207-213.doi: 10.16265/j.cnki.issn1003-3033.2022.10.2123

二甲双胍改善瓦斯爆炸肾损伤的试验研究

田林强¹(), 来枫¹, 张玥¹, 翁孝刚¹, 姚三巧², 任文杰¹^,^**()

¹ 新乡医学院第三附属医院创伤与骨科研究所，河南新乡 453003
² 新乡医学院公共卫生学院，河南新乡 453003

收稿日期:2022-04-14 修回日期:2022-08-11 出版日期:2022-10-28 发布日期:2023-04-28
通讯作者: 任文杰
作者简介:
田林强（1982—），男，河南汝州人，博士，副教授，主要从事煤矿瓦斯爆炸伤的致伤机制及干预措施方面的研究。E-mail：wstiansenzhi@126.com。
翁孝刚教授
姚三巧教授
基金资助:
中国职业安全健康协会创新创业项目(CXCY-2021-11); 新乡医学院第三附属医院开放课题项目(KFKTZD202104)

Experimental study on treatment effect of MET on renal injuries in gas explosion

TIAN Linqiang¹(), LAI Feng¹, ZHANG Yue¹, WENG Xiaogang¹, YAO Sanqiao², REN Wenjie¹^,^**()

¹ Institute of Trauma and Orthopedics， Third Affiliated Hospital of Xinxiang Medical University， Xinxiang Henan 453003， China
² School of Public Health， Xinxiang Medical University， Xinxiang Henan 453003， China

Received:2022-04-14 Revised:2022-08-11 Online:2022-10-28 Published:2023-04-28
Contact: REN Wenjie

摘要/Abstract

摘要：

为明确瓦斯爆炸肾损伤的特点以及二甲双胍（MET）的治疗效果，并探讨其可能的作用机制，将大鼠随机分为正常对照组（NC组）、瓦斯爆炸肾损伤组（SW组）和瓦斯爆炸肾损伤+MET治疗组（MET组），利用真实巷道建立瓦斯爆炸大鼠肾损伤模型，检测肾功能，观察肾脏组织学变化及凋亡相关分子的表达变化，并检测腺苷酸活化蛋白激酶（AMPK）和PGC-1α的表达情况。结果表明：瓦斯爆炸肾损伤组织学表现为肾小球和肾小管明显充血和水肿，伴肾功能异常；MET能够改善肾功能，减轻肾损伤，可能是通过激活AMPK及其下游分子PGC-1α，抑制细胞凋亡实现的。

关键词: 二甲双胍（MET）, 瓦斯爆炸, 急性肾损伤, 腺苷酸活化蛋白激酶（AMPK）, 过氧化物酶增殖物激活受体γ共激活因子1α（PGC-1α）

Abstract:

In order to explore characters of kidney injuries caused by gas explosion and treatment effect of MET as well as its possible action mechanism， rat samples were divided into normal control group （NC group）， kidney injury caused by gas explosion group （SW group） and kidney injury caused by gas explosion treated with MET group randomly. Then， kidney injury model of rats was built by utilizing actual roadway， renal functions were tested， histological changes of kidney and express changes of apoptosis associated molecular were observed， and those of AMPK and PGC-1α were detected. The results show that kidney injuries are normally expressed as significant congestion and edema in renal corpuscle and kidney tubules along with kidney disfunction. MET can improve renal function and decrease damages probably through activating AMPK and PGC-1α， thus inhibiting apoptosis of renal tubular epithelial cells.

Key words: metformin（MET）, gas explosion, acute kidney injury, AMP-activated protein kinase （AMPK）, peroxidase proliferator-activated receptor gamma coactivator 1α （PGC-1α）

田林强, 来枫, 张玥, 翁孝刚, 姚三巧, 任文杰. 二甲双胍改善瓦斯爆炸肾损伤的试验研究[J]. 中国安全科学学报, 2022, 32(10): 207-213.

TIAN Linqiang, LAI Feng, ZHANG Yue, WENG Xiaogang, YAO Sanqiao, REN Wenjie. Experimental study on treatment effect of MET on renal injuries in gas explosion[J]. China Safety Science Journal, 2022, 32(10): 207-213.

图/表 4

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二甲双胍改善瓦斯爆炸肾损伤的试验研究

Experimental study on treatment effect of MET on renal injuries in gas explosion

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